Cloning and Expresssion Analysis of bZIP Gene from Pyrus pyrifolia ‘Huanghua’

doi:10.3969/j.issn.1005-3395.2014.02.010

Home > Archive>Volume 22, Issue 2, 2014 >172-178. DOI:10.3969/j.issn.1005-3395.2014.02.010

Cloning and Expresssion Analysis of bZIP Gene from Pyrus pyrifolia ‘Huanghua’
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                        10.3969/j.issn.1005-3395.2014.02.010
                    
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Affiliation:Luzhou Agriculture Bureau,Fujian Agriculture and Forestry University, College of Horticulture,Fujian Agriculture and Forestry University, College of Horticulture,Fujian Agriculture and Forestry University, College of Horticulture,Fujian Agriculture and Forestry University, College of Horticulture
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Abstract:

In order to understand the function of PpbZIP of Pyrus pyrifolia ‘Huanghua’, PpbZIP gene was cloned from flower buds of ‘Huanghua’ based on homologous gene of bZIP. The results showed that full length of cDNA of PpbZIP (GenBank accession No.: KC951877) was 1843 bp, containing an ORF of 1227 bp and encoding 408 amino acids. PpbZIP also shared 92% homology with bZIP of Malus × domestica. QRT-PCR analysis showed that the expression of PpbZIP gene increased at initiate dormancy stage and then decreased with dormancy releasing. It was suggested that PpbZIP could involve in the regulation of bud dormancy process of ‘Huanghua’.

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宋燕春,李永裕,黄添毅,陈铁娇,吴少华.黄花梨bZIP的cDNA克隆与表达分析[J].热带亚热带植物学报,2014,22(2):172~178

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Received:May 07,2013
Revised:August 03,2013
Adopted:October 31,2013
Online: March 26,2014
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