Cloning and Expression Characterization of Histone Deacetylase Gene HDA705 Promoter in Rice (Oryza sativa)

Abstract:

In order to understand the function and expression character of histone deacetylase gene HDA705 in rice (Oryza sativa), 5′ terminal promoter sequence was cloned with length of 2 kb based on rice HDA705 sequence (GenBank accession No.: AK111861), named proHDA705. The expression vector of proHDA705:GUS was constructed. The proHDA705:GUS vector was transformed to rice by Agrobacterium-mediated assays. GUS staining assays of transgenic rice showed that proHDA705 expressed only in root, stem, leave and some epidermal hair of seed coat, but did not in flower. It was suggested that HDA705 had tissue specific expression in rice.