Home > Archive>Volume 21, Issue 5, 2013 >439-445. DOI:10.3969/j.issn.1005-3395.2013.05.010
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Isolation and Expression of Phytoene Synthase Gene in Gardenia jasminoides
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Guangdong Pharmaceutical University,Guangdong Pharmaceutical University

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    Abstract:

    In order to understand the synthesis mechanism of crocin in Gardenia jasminoides fruits, the full-length cDNAs of phytoene synthase gene GjPSY from G. jasminoides were cloned. The results showed that the deduced amino acid sequence of GjPSY had close relation with those from dicotyledonsous plants. The content of crocin-1 in fruits was (3.96±1.48) mg g-1 by HPLC, while crocin-1 was not detected in leaves. It was revealed that crocin-1 exclusively accumulated in fruits. The RT-PCR analysis revealed that the expression of GjPSY in fruits was not differences from that in leaves. Therefore, it was suggested that the crocin synthesis in G. jasminoides fruits had not correlation with transcription level of GjPSY gene.

    Reference
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高蓝,朱碧云.栀子八氢番茄红素合成酶基因的分离及表达分析[J].热带亚热带植物学报,2013,21(5):439~445

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History
  • Received:March 06,2013
  • Revised:May 02,2013
  • Adopted:June 13,2013
  • Online: September 23,2013
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