Abstract:The aim was to understand the function of mitochondrial fission proteins. Two homologues, NtFIS1A and NtFIS1B, were identified by searching tobacco (Nicotiana tabacum) EST database with mitochondiral fission member of FIS1A and FIS1B in Arabidopsis. Expression of NtFIS1A/1B in tobacco ‘BY-2’ suspension cells were inhibited by using RNA interference and artificial microRNA interference. The RT-PCR results showed that the transcription of NtFIS1A/1B were knockdown in related RNAi cell lines. Thereafter, mitochondrial morphology in RNA knockdown cell lines was analyzed. The average area of single mitochondria significantly increased. It was concluded that NtFIS1A/1B participated in mitochondrial fission in ‘BY-2’ suspension cells. It would contribute to understand morphological regulation of mitochondria in tobacco suspension cells.
