ISSR Analysis of Melastoma Germplasm in Fujian Province

Abstract:

In order to understand the relationship of Melastoma, the DNA fingerprints of 34 Melastoma germplasm collected from different regions of Fujian Province were studied by using ISSR markers. Eleven primers selected from 116 ISSR primers could obtain high polymorphism and reproducibility bands. The results showed that total of 112 DNA bands were amplified, and 104 polymorphic bands, counting for 92.9%. Genetic similarity analysis showed that for 34 Melastoma germplasm the Nei's coefficient of 34 Melastoma germplasm ranged from 0.55 to 0.93, with the average of 0.71. It was suggested that there were close genetic relationships and high genetic diversity among Melastoma germplasm resources. These 34 germplasm resources could divided into 3 groups and 5 subgroups by UPGMA analysis, and 4 groups by principal coordinate scatter analysis. These results had high consistency with that of genetic relationship analysis. Therefore, it was suggested that ISSR markers could be used to reveal genetic diversity of Melastoma at molecular level, and provided a theoretical basis for breeding of Melastoma in Fujian.

Key words:Melastoma;Genetic relationship;Molecular marker;Germplasm;Fujian Province