Studies on Genetic Diversity for Specialty Kiwifruits from Southwestern Regions of China Using AFLP System

doi:10.3969/j.issn.1005-3395.2013.04.004

Home > Archive>Volume 21, Issue 4, 2013 >315-322. DOI:10.3969/j.issn.1005-3395.2013.04.004

Studies on Genetic Diversity for Specialty Kiwifruits from Southwestern Regions of China Using AFLP System
DOI:
                        10.3969/j.issn.1005-3395.2013.04.004
                    
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Author:
                        QIN Xiao-boQIN Xiao-bo
1. Sichuan Academy of Natural Resource Sciences, China;2. Sichuan Provincial Laboratory for Natural Resources Protection and Sustainable Utilization, China;3. College of Life Science, Sichuan University, China
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GAO Ji-haiGAO Ji-hai
1. College of Life Science, Sichuan University, China;2. Chengdu University of Traditional Chinese Medicine, China
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Affiliation:1.Sichuan Academy of Natural Resource Sciences，2.College of Life Sciences, Sichuan University,2. College of Life Sciences, Sichuan University，3.Pharmacy College, Chengdu University of Traditional Chinese Medicine
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Abstract:

In order to understand the genetic diversity of kiwifruits in southwest of China, AFLP system for kiwifruits was optimized. The optimum AFLP system was constructed as following: 300 ng DNA was digested with EcoR I/Mse I (15 U/5 U) at 37℃ for 2 h, diluted by 5-fold after ligating with adaptors for the preamplification, and then the production of pre-amplification diluted 10-fold was used for selective amplification. The results showed that 979 electrophoretic bands were identified totally, of which 649 bands were polymorphic by using 22 pairs of primers. The genetic diversity of ten kiwifruit species, including Actinidia chinensis, A. deliciosa, A. chinensis var. hispida, A. venosa fo. pudescens, A. rubricaulis var. coriacea, A. tetramera, A. kolomikta, A. ploygama, A. callosa var. henryi and A. arguta var. purpurea, from southwestern China, were analysis by using the optimum AFLP system. The results indicated that there were obvious clustering relationship among intraspecific and interspecific, and cross cluster existed in species between sect. Maculatae and sect. Leiocarpae, related to geographic regional distribution.

Key words:Kiwifruit;AFLP;Genetic diversity;Southwestern China;Geographic region

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秦小波,高继海.利用AFLP分析西南特色猕猴桃的遗传多样性[J].热带亚热带植物学报,2013,21(4):315~322

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Received:November 26,2012
Revised:March 26,2013
Adopted:April 24,2013
Online: July 19,2013
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