Abstract:In order to establish suspension cell system of Mirabilis jalapa L., the calli induced from leaves of aseptic seedlings of M. jalapa were used as material, the optimum suspension culture system was studied. The results showed that the stable suspension cell system could be obtained after calli were subcultured 3-4 times on MS medium with 2,4-D 1 mg L-1 and KT 0.5 mg L-1. The optimum pH was 5.5-5.9 with 30 g L-1 sucrose. The growth curve of suspension cells of M. jalapa was S-type, and subculture time was about 10 days. The optimum inoculum was 7.5 mL in 40 mL medium. Secretory protein was (0.42±0.15) g extracted from 1 L medium. These were useful for studying secretory protein extracted from suspension system.