Home > Archive>Volume 21, Issue 1, 2013 >29-37. DOI:10.3969/j.issn.1005–3395.2013.01.004
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Incomplete Concerted Evolution of nrDNA ITS in Cornus subg. Syncarpea (Cornaceae)
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South China Botanical Garden,Department of Plant Biology, North Carolina State Universiy,South China Botanical Garden

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    Abstract:

    The subgenus Syncarpea, which belongs to genus of Cornus (Cornaceae), includes five species and eight subspecies in China. nrDNA ITS sequences of 21 individuals from 4 species, with 1 to 2 populations for each species, in the subgenus were amplified and used to test the existence of incomplete concerted evolution and pseudogenes. Intra-genomic polymorphism was detected in the internal transcribed spacer (including ITS1, 5.8S and ITS2) regions of these species. The analyses of G+C content, 5.8S conserved motifs and minimum free energy of 5.8S secondary structures suggested that there were putative pseudogenes of nrDNA in these species. Phylogenetic analyses of the sequences resulted in five clades with variants from the same accession commonly being placed in two or more clades and species were grouped differently among clades. These results demonstrated the existence of incomplete concerted evolution of ITS within individuals of Syncarpea, which could be explained by incomplete lineage sorting, possible hybridization or polyploidy and resulted in the polymorphism of ITS and the difficulty in morphology-based species delineation in the subgenus.

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胡兆锋,向秋云,黄宏文.四照花亚属的nrDNA ITS致同进化不完全[J].热带亚热带植物学报,2013,21(1):29~37

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History
  • Received:May 08,2012
  • Revised:June 11,2012
  • Adopted:June 18,2012
  • Online: January 17,2013
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