Home > Archive>Volume 21, Issue 3, 2013 >253-258. DOI:10.3969/j.issn.1005-3395.2013.03.011
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Establishment and Application of SCoT Amplification System for Macadamia
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Guangxi Subtropical Crops Research Institute

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    Abstract:

    SCoT-PCR amplification system of macadamia (Macadamia spp.) was optimized and established by using single factor design, such as Mg2+, dNTPs, primer, Taq DNA polymerase and DNA template, and DNA fingerprint maps of macadamia germplasms were constructed. The results showed that final volume of optimum reaction system was 20 μL, including 2.5 mmol L-1 Mg2+, 0.3 mmol L-1 dNTPs, 0.8 μmolL-1 primer, 1.0 U Taq DNA polymerase, 40 mgL-1 template DNA, and 2 μL 10 × buffer with ddH2O water. The suitable annealing temperature of primers was 50℃. The optimum SCoT-PCR system was shown to be steady and reliable. The 12 germplasms of macadamia could be absolutely identified by primer SC5 and SC34 + SC39 with 99.988% probability of confidence, every germplasm of macadamia had a unique fingerprint map. It showed that the optimum SCoT-PCR system could be applied effectively in germplasm identification and genetic diversity analysis of macadamia.

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蔡元保,杨祥燕,陈显国,曾黎明,郭凌飞,林玉虹,崔明勇.澳洲坚果SCoT反应体系的建立及应用[J].热带亚热带植物学报,2013,21(3):253~258

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History
  • Received:February 27,2012
  • Revised:April 27,2012
  • Adopted:July 23,2012
  • Online: May 22,2013
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