Home > Archive>Volume 20, Issue 5, 2012 >469-474. DOI:10.3969/j.issn.1005-3395.2012.05.007
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Cloning and Expression Analysis of HbNAC1 in Hevea brasiliensi
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1College of agriculture,Hainan University; 2The Institute of Tropical Bioscience and Biotechnology,Chinese academy of tropical agricultural sciences,Hainan academy of agricultural sciences

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    Abstract:

    Based on EST sequences from an ethephon-induced latex SSH cDNA library of Hevea brasiliensis, a full-length cDNA, named as HbNAC1, was cloned from H. brasiliensis by RACE-PCR, encoding a NAC transcription factor. The full-length of HbNAC1 cDNA was 1419 bp with an open reading frame (ORF) of 930 bp, and encoding 309 amino acids. The deduced HbNAC1 with NAM domains had the typical characteristics of NAC family, and homologous to NACs in Populus trichocarpa, Ricinus communis, Oryza sativa and Arabidopsis thaliana of 79%, 80%, 57% and 60%, respectively. Phylogenetic analysis showed that HbNAC1 was classified into the Ⅱ subfamily of NAC family. Semi-quantitative RT-PCR analysis indicated that the expression of HbNAC1 was rich in latex, and significantly enhanced by ethephon stimulation.

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邱琼,朱家红,张治礼.巴西橡胶树HbNAC1基因的克隆和表达分析[J].热带亚热带植物学报,2012,20(5):469~474

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History
  • Received:November 21,2011
  • Revised:February 14,2012
  • Adopted:May 22,2012
  • Online: September 26,2012
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