Cloning and Expression Analysis of Cytosolic Malate Dehydrogenase Gene from Sugarcane

Abstract:

A full-length cDNA sequence of sugarcane (Saccharum officinarum L.) cytosolic malate dehydrogenase (cMDH) gene, named as Sc-cMDH, was obtained from sugarcane full-length cDNA library by EST sequencing and bioinformatics analysis. The full-length of Sc-cMDH was 1314 bp with 999 bp open reading frame, encoding 332 amino acids. Sc-cMDH shared 86.5%~97.0% of amino acids with cMDH from other 9 species. Sc-cMDH contains typical NAD⁺ binding motif (T¹¹GAAGQI¹⁷) and catalytic motif (I¹⁸⁴WGNH¹⁸⁸). Real-time PCR analysis showed that the expression of Sc-cMDH was higher in leaves and roots than that in stems of sugarcane.