Home > Archive>Volume 20, Issue 5, 2012 >455-461. DOI:10.3969/j.issn.1005-3395.2012.05.005
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Cloning and Expression Analysis of Cytosolic Malate Dehydrogenase Gene from Sugarcane
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Fujian Agriculture and Forestry University, Sugarcane Institute,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University,ife Science College, Fujian Agriculture and Forestry University,Genetic Institute of Rice, Fujian Agriculture and Forestry University,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University

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    Abstract:

    A full-length cDNA sequence of sugarcane (Saccharum officinarum L.) cytosolic malate dehydrogenase (cMDH) gene, named as Sc-cMDH, was obtained from sugarcane full-length cDNA library by EST sequencing and bioinformatics analysis. The full-length of Sc-cMDH was 1314 bp with 999 bp open reading frame, encoding 332 amino acids. Sc-cMDH shared 86.5%~97.0% of amino acids with cMDH from other 9 species. Sc-cMDH contains typical NAD+ binding motif (T11GAAGQI17) and catalytic motif (I184WGNH188). Real-time PCR analysis showed that the expression of Sc-cMDH was higher in leaves and roots than that in stems of sugarcane.

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徐景升,阙友雄,颜克伟,唐唯其,许莉萍,高三基,郭晋隆,董萌,陈如凯.甘蔗细胞质型苹果酸脱氢酶基因的克隆及其表达分析[J].热带亚热带植物学报,2012,20(5):455~461

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History
  • Received:November 14,2011
  • Revised:March 03,2012
  • Adopted:April 25,2012
  • Online: September 26,2012
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