GAO He-qiong
1. Rubber Research Insititute, Chinese Academy of Tropical Agricultural Sciences, China;2. College of Agriculture, Hainan University, ChinaZHUANG Nan-sheng
College of Agriculture, Hainan University, ChinaWANG Ying
College of Agriculture, Hainan University, ChinaQIU Hai-yan
College of Agriculture, Hainan University, ChinaThe in situ PCR technical system on chromosomes of Hevea brasiliensis was developed successfully. The HbMyb1 gene related with Tapping Panel Dryness (TPD) resistance was physically located in the long arm of chromosome No. 5 of ‘Reyan 7-33-97’ by in situ PCR and the fraction-length, the percentage of the distance from detection site to the centromere, was 15.21. One or two signals were detected during cell division at different phases. The chromosome preparations, preservation, and pretreatment were also discussed.
高和琼,庄南生,王英,邱海燕.巴西橡胶树HbMyb1基因的原位PCR物理定位[J].热带亚热带植物学报,2012,20(4):365~368
Copy
