ZHANG Quan-qi
1. Guangdong State Farms Tropical Crop Research Institute, China;2. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, ChinaNI Yan-mei
Guangdong State Farms Tropical Crop Research Institute, ChinaZHU Jia-hong
Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, ChinaHUANG Zhi
Guangdong State Farms Tropical Crop Research Institute, ChinaUsing RACE and RT-PCR techniques, a novel member of WRKY gene family, named as HbWRKY1, was isolated from H. brasiliensis. Bioinformatics analysis showed that the full length cDNA of HbWRKY1 was 1234 bp, containing a 912 bp open reading frame and encoding 303 amino acids. The deduced amino acid sequence was highly homologous to WRKY proteins from Ricinus communis and Populus tremula × P. alba by 79% and 73%, respectively. The HbWRKY1 was constructed into pCAMBIA1304 expression vector, and transformed into tobacco (Nicotiana tabacum) mediated by Agrobacterium. Sixty-five hygromycin B (Hyg) resistant regenerated plantlets of tobacco were selected. PCR detection indicated that all the Hyg resistant tobacco plants contained the alien HbWRKY1 gene. It confirmed that the over-expression of HbWRKY1 under drought stress could obviously enhanced drought-tolerance of transgenic tobacco plantlets. Therefroe, it suggested that there were relationship between WRKY gene and drought tolerance of Hevea brasiliensis.
张全琪,倪燕妹,朱家红,黄志.巴西橡胶树HbWRKY1基因的克隆及转化烟草的初步研究[J].热带亚热带植物学报,2012,20(4):356~364
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