Abstract:The Effects of sampling schemes on construct method for core collection of Canarium album L. based on molecular markers data were studied on the basis of ISSR results of 64 germplasm materials of C. album from eastern Guangdong, and using UPGMA cluster method according to SM and Nei & Li genetic distance by stepwise clustering. The impact of sampling strategies, such as P, S, L and G, on the construction of core collection with different groupings was discussed. Compared with the number of polymorphic loci, percentage of polymorphic loci, observed number of alleles, effective number of alleles, Nei’s genetic diversity index and Shannon’s information index of different sample groups, 16 samples were finally chosen as the core collection according to genetic proportion strategy based on Nei & Li genetic distance. The core collection retained 25% of germplasm of the initial samples, and the retention ratio of the number of polymorphic loci and percentage of polymorphic loci was 92.93% and 98.31%, respectively, and the observed number of alleles, effective number of alleles, Nei’s genetic diversity index and Shannon’s information index reached 99.26%, 102.56%, 107.39% and 106.29%, respectively. It suggested that the core collection constructed by this method could goodly represent the genetic diversity of initial germplasm bank.
