Abstract:Six compounds were isolated from the EtOH extract of Manihot esculenta (cassava) stems. On the basis of spectral data, they were identified as furan (1), ceplignan (2), indole-3-carboxylic acid (3), 3,9,13-megastigmanetriol (4), amentoflavone (5) and yucalexin P-21 (6). Compounds 1-5 were obtained from cassava for the first time. Antibacterial activity was assayed by paper disk diffusion method, and compounds 1,3,4, and 6 showed inhibitory activity against methicillin-resistant Staphylococcus aureus (MRSA) and S. aureus.