Abstract:A fungal strain of Acremonium sp. SC0105 was obtained from tissue culture of humus collected at Dinghushan, Guangdong Province, China. The EtOH extract of this fungal strain showed antibacterial activity against Staphylococcus aureus. From the solid cultures of this fungus, five compounds were isolated by repeated column chromatography. On the basis of their spectral data, they were identified as gingerglycolipid B (1), gingerglycolipid C (2), D-Mannitol (3), flazine (4), and acremine F (5). Among them, flazine (4) was isolated from fungus of Acremonium genus for the first time.