Home > Archive>Volume 19, Issue 6, 2011 >506-512. DOI:10.3969/j.issn.1005-3395.2011.06.003
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Genetic Diversity of Miscanthus floridulus ( Poaceae ) from Guangdong by Intersimple Sequence Repeat ( ISSR )
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    Abstract:

    The genetic diversity of 215 individuals of Miscanthus floridulus from 13 natural populations distributed in Guangdong Province was investigated by using intersimple sequence repeat (ISSR) techniques. A total of 81 unambiguous and repetitious bands were scored by 9 primers. The results showed that the genetic diversity of M. floridulus was high, at species level, percentage of polymorphic loci (PPB) was 93.83%, Nei's gene diversity (He) for 0.3969, and Shannon's information index (Hsp) for 0.5724; while at population level, PPB=81.91%, He=0.3015 and Shannon's information index (Hpop)=0.4460. The coefficient of genetic differentiation among populations (Gst) was 0.2379, and gene flow among populations (Nm) was 1.6018. The AMOVA analysis showed that 79.56% of the total genetic variation within populations and the rest (20.44%) partitioned among populations. No significant correlation was found between geographical and genetic distance (r=-0.2811, P=0.9590).

    Reference
    [1] Liou L(刘亮). Flora Reipublicae Popularis Sinicae Tomus 10(2) [M]. Beijing: Science Press, 1997: 3-11.(in Chinese)
    [2] Emily A H, Frank G, Dohleman W, et al. Meeting US biofuel goals with less land: The potential of Miscanthus [J]. Glob Change Biol, 2008, 14(9): 2000-2014.
    [3] Heaton E A, Clifton-Brown J C, Voigt T B, et al. Miscanthus for renewable energy generation: European union experience and projections for Illinois [J]. Mit Adapt Strat Glob Change, 2004, 9(4): 433-451.
    [4] Clifton-Brown J C, Lewandowski I, Andersson B, et al. Performance of 15 Miscanthus genotypes at five sites in Europe [J]. Agron J, 2001, 93(5): 1013-1019.
    [5] Walsh M. Miscanthus Handbook:EU project FAIR 3-CT96-1707 [M]. Cork: Hyperion, 1997: 1-4.
    [6] Zeng X L(曾宪录), Liao F L(廖富林), Wen G R(温冠儒), et al. Investigation of the distribution and growth of major energy grasses in Meizhou City [J]. Guangdong Agri Sci(广东农业科学), 2008(7): 25-28.(in Chinese)
    [7] Xu Z R(徐泽荣), Yang L(杨林). Plant resources and its potential future for exploitation of Miscanthus in Sichuan [J]. Pruat Anim Husb(草业与畜牧), 2009(9): 22-27,54.(in Chinese)
    [8] Xiao Y F(萧运峰), Gao J(高洁). A comparative study on differentiated types and productive performance of Miscanthus floridulus [J]. J Sichuan Grass(四川草原), 1998(1): 21-23,50.(in Chinese)
    [9] Chen H J(陈慧娟), Zhang Z W(张卓文), Ning Z L(宁祖林), et al. Effect of fertilization on the caloric value and morphological properties of Miscanthus floridulus [J]. Prat Tural Sci(草业科学), 2009, 26(8): 63-67.(in Chinese)
    [10] Chen S F(陈少风), He J(何俊), Zhou P H(周朴华), et al. The karyorypes of Miscanthus sinensis and M. floridulus [J]. Acta Agri Univ Jiangxi(江西农业大学学报), 2008, 30(1): 123-126.(in Chinese)
    [11] Jin L(金琳), Ge G(葛刚), Chen S F(陈少风), et al. Optimization of ISSR-PCR reaction system for Miscanthus floridulus [J]. J Anhui Agri Sci(安徽农业科学), 2009, 37(29): 14041-14043,14077.(in Chinese)
    [12] Hu H K(胡恒康), Jiang X M(江香梅), Huang J Q(黄坚钦), et al. Tissue culture and rapid propagation of Miscanthus floridulus (Labnll.) Warb [J]. Plant Physiol Commun(植物生理学通讯), 2009, 45(11): 1109.(in Chinese)
    [13] Diao Y(刁英), Hu X H(胡小虎), Zheng X F(郑兴飞), et al. Analysis of genetic diversity in Miscanthus floridulus using SRAP and ISSR marker [J]. J Wuhan Univ (Nat Sci)(武汉大学学报: 理学版), 2010, 56(5): 578-583.(in Chinese)
    [14] Liu L(刘莉), Deng C T(邓春婷), Bao M Z(包满珠). Analysis of genetic diversity within the population of buffalo grass by morphological traits and ISSR markers [J]. Prat Tural Sci(草业科学), 2008, 25(1): 100-106.(in Chinese)
    [15] Fan Y(范彦), Li F(李芳), Zhang X Q(张新全), et al. Genetic diversity of Hemarthria compressa germplasm detected by inter-simple sequence repeat (ISSR) [J]. Acta Prat Sin(草业学报), 2007, 16(4): 76-81.(in Chinese)
    [16] Zeng B(曾兵), Zhang X Q(张新全), Fan Y(范彦), et al. Genetic diversity of Dactylis glomerata germplasm resources detected by intersimple sequence repeats (ISSRs) molecular markers [J]. Hereditas(遗传), 2006, 28(9): 1093-1100.(in Chinese)
    [17] Li A, Ge S. Genetic variation and diversity of Psammochloa villosa (Poaceae) detected by ISSR markers [J]. Ann Bot, 2001, 87(5): 585-590.
    [18] Li Y X(李永祥), Li S S(李斯深), Li L H(李立会), et al. Comparison of genetic diversity of twelve Elymus species using ISSR and SSR markers [J]. Sci Agri Sin(中国农业科学), 2005, 38(8): 1522-1527.(in Chinese)
    [19] Doyle J. DNA protocols for plants: CTAB total DNA isolation [M]// Hewitt G M, Johnston A. Molecular Techniques in Taxonomy. Berlin: Springer, 1991: 283-293.
    [20] Ausubel F M, Brent R, Kingston R E, et al. Short Protocols in Molecular Biology [M]. Indianapolis: John Wiley & Sons, Inc, 1995: 2-11.
    [21] Yeh F C, Yang R C, Boyle T B J, et al. POPGENE: The User-friendly Shareware for Population Genetic Analysis [CP/DK]. Release 1.31, Canada, Edmonton, University of Alberta, Molecular Biology and Biotechnology Centre, 1997.
    [22] Nei M. Analysis of gene diversity in subdivided populations [J]. Proc Natl Acad Sci USA, 1973, 70(12): 3321-3323.
    [23] Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases [J]. Proc Natl Acad Sci USA, 1979, 76(10): 5269-5273.
    [24] Rohlf F J. NTSYSpc: Numerical Taxonomy and Multivariate Analysis System Version 2.1 [DB/CD]. New York, USA: Setauket, Exeter Software, 2000.
    [25] Excoffier L, Smouse P E, Quattro J M. Analysis of molecular variance inferred from metric distances among DNA hapltypes: Applications to human mitochondrial DNA restriction data [J]. Genetics, 1992, 131: 479-491.
    [26] Zhang F M(张富民), Ge S(葛颂). Data analysis in population genetics:Ⅰ. Analysis of RAPD data with AMOVA [J]. Biodiv Sci(生物多样性), 2002, 10(4): 438-444.(in Chinese)
    [27] Miller M P. Tools for Population Genetic Analysis (TEPGA): Version 1.3 [CP/DK]. Arizona: Northern Arizona University, Department of Biological Sciences, 1997.
    [28] Hamrick J L, Godt M J W. Allozyme diversity in plant species [M]// Brown A D H, Clegg M T, Kahler A L, et al. Plant Population Genetics, Breeding and Genitic Resources. Sunderland: Sinauer Press, 1989: 134-619.
    [29] Korron J D. A comparison of levels of genetic polymorphism and self-compatibility: Geographically restricted and widespread plant congeners [J]. Ecology, 1987, 1(1): 47-58.
    [30] Hamrick J L, Godt M J W, Sherman-Broyles S L. Factors influencing levels of genetic diversity in woody plant species [J]. New For, 1992, 6(1): 95-124.
    [31] Hamrick J L, Godt M J W. Effects of life history traits on genetic diversity in plant species [J]. Phil Trans Roy Soc London Biol Sci, 1996, 351(1345): 1291-1298.
    [32] Bussell J D. The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeiaceae) [J]. Mol Ecol, 1999, 8(5): 775-789.
    [33] Hogbin P M, Peakall R. Evaluation of the contribution of genetic research to the management of the endangered plant Zieria prostrate [J]. Cons Biol, 1999, 13(3): 514-522.
    [34] Loveless M D, Hamrick J L. Ecological determinants of genetic structure in plant populations [J]. Ann Rev Ecol, 1984(15): 65-95.
    [35] Hartl D L, Clark A G. Principle of Population Genetics [M]. 2nd ed. Sunderland: Sinauer Press, 1989: 1-372.
    [36] Ge S(葛颂). Application of DNA molecular marker in conservation biology [M]// Zou Y P(邹喻苹), Ge S(葛颂), Wang X D(王晓东). Molecular Markers in Systematic and Evolutionary Botany. Beijing: Science Press, 2001: 140-149.(in Chinese)
    [37] Kumar A, Rogstad S H. A hierarchical analysis of microsatellite DNA diversity in Gambel oak (Quercus gambelii Nutt.; Fagaceae) [J]. Mol Ecol, 1998, 7(7): 859-869.
    [38] Slatkin M, Borton N H. A comparison of three indirect methods for estimating average levels of gene flow [J]. Evolution, 1989, 43(7): 1349-1368.
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李婧,夏念和.广东省五节芒遗传多样性的ISSR分析[J].热带亚热带植物学报,2011,19(6):506~512

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History
  • Received:April 07,2011
  • Revised:May 15,2011
  • Adopted:May 23,2011
  • Online: November 18,2011
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