Abstract:PsbS protein plays an important role in non-photochemical quenching (NPQ) in plants. A PsbS gene was cloned from Moso bamboo (Phyllostachys edulis) leaves by RT-PCR method, which named PePsbS (GenBank No. FJ600727) with 810 bp in length, and encoded a polypeptide with 269 amino acids. Homology analysis showed that the deduced PsbS was highly homologous to those from other monocotyledon plants. The protein structure analysis indicated that PePsbS consists of transit peptide and mature protein including four transmembrane domains. The fragment of PePsbS gene encoding mature protein was constructed in multiple cloning site of pET23a and expressed in Escherichia coli induced by IPTG. The expression of PePsbS was the best under 41℃ for 4 hours, the recombinant protein accounted for 21.5% of the total proteins with molecular weight about 22 kD. It indicated that there were significant effects of temperature and IPTG induced time on protein expression.
