LI Yu-zhong
1. School of Life Sciences, Yunnan University, China; 2. Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, ChinaSHEN Fei
1. School of Life Sciences, Yunnan University, China; 2. Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, ChinaFAN Jing
School of Life Sciences, Yunnan University, ChinaWANG Xiao-xia
School of Life Sciences, Yunnan University, ChinaLI Jun-ying
School of Life Sciences, Yunnan University, ChinaWANG Jian-guang
School of Life Sciences, Yunnan University, ChinaCHEN Sui-yun
School of Life Sciences, Yunnan University, ChinaThe spore development and callus induction of Bryum dichotomum Hedw. in vitro culture were studied. The results showed that the spore germination and protonema development of B. dichotomum Hedw. were Bryum-type. The calli were successfully induced from protonema cultured on solid MS medium supplemented with 3.0 mg L-1 6-BA and 2.0 mg L-1 sodium silicate. The calli could be subcultured at long term on solid MS medium supplemented with 2.0 mg L-1 sodium silicate, 1.0 mg L-1 2,4-D and 1.0 mg L-1 6-BA. The fresh weight of calli cultured for 28 days was about 9.25 times to original weight under suspension culture, when incubated in liquid MS medium supplemented with 2.0 mg L-1 sodium silicate, 1.0 mg L-1 2,4-D and 1.0 mg L-1 6-BA.
李育中,沈霏,范静,王晓霞,李骏盈,王建光,陈穗云.双色真藓的组织培养和发育研究[J].热带亚热带植物学报,2012,20(1):39~43
Copy
