Callus Induction and Establishment of Suspension Cell Line of Ardisia crenata Sims.

Abstract:

Stems, leaves, hypocotyls and radicles from aseptic seedlings of Ardisia crenata Sims. used as explants were cultured on different mediums to induce callus. The results showed that radicles of A. crenata cultured on mediums with 2,4-D had the highest callus inducation rate. The callus subcultured on MS +2,4-D 0.5 mg L^-1+KT 0.01 mg L^-1 supplemented with 5 mg L^-1AgNO₃ had more than 8 times proliferation coefficient. Five types of callus, such as white, wet and soft callus (I), white gibraltar-like callus (II), yellow granular callus (III), yellow green nubbly callus (IV), and green nubbly callus (V) were obtained. Among which callus II and III could successfully establish suspension cell system, and the callus could be well maintained cultured on MS+2,4-D 0.5 mg L^-1+KT 0.01 mg L^-1 by solid-liquid transition.