YU Feng
1. College of Life Sciences, Wenzhou Medical College, China; 2. Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, ChinaZHENG Chun-fang
Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, ChinaSHI Meng-ru
College of Life Sciences, Wenzhou Medical College, ChinaLIU Wei-cheng
Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, ChinaQIU Jian-biao
Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, ChinaJI De-wei
Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, ChinaHUANG Li
Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, ChinaCHEN Shao-bo
Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, ChinaIn order to study the proteome of Kandelia candel leaves, a two-dimensional electrophoresis (2-DE) system was established and optimized. Compared with the low protein content (1.7 μg μL-1) extracted by phenol (Phe), the improved trichloroacetic acid/acetone precipitation (TCA) method could obviously increase the extracting efficiency up to 12.4 μg μL-1. The protein spots of Kandelia candel leaves mainly distributed in the range of pH 4-7. The optimal loading quantity of samples were 400 μg gel-1 for a 17 cm IPG strip (pH 3-10 NL), which can detect 564±34 protein spots after silver staining. Therefore, the high resolution and repeatable 2-DE map could be obtained by using TCA extract method and the optimum system, which could widely use in proteome study of Kandelia candel.
于冯,郑春芳,施孟如,刘伟成,仇建标,冀德伟,黄丽,陈少波.红树植物秋茄叶片双向电泳技术体系的建立及优化[J].热带亚热带植物学报,2011,19(6):519~523
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