Home > Archive>Volume 19, Issue 5, 2011 >400-406. DOI:10.3969/j.issn.1005-3395.2011.05.002
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Screening Interaction Proteins of Rubber Elongation Factor (REF) by Yeast Two-hybrid System
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    Abstract:

    The interaction proteins of rubber elongation factor (REF) were screened using yeast two-hybrid system. A bait vector pBD-GAL4-REF without auto-activation was constructed by subcloning ORF cDNA of REF gene into the yeast two-hybrid vector pBD-GAL4, and the pAD-GAL4-2.1 cDNA prey library was prepared with mRNA extracted from the latex of rubber tree (Hevea brasiliensis). The bait and prey vectors were then co-transformed into YRG-2 yeast competent cells by two-step method. Five proteins interacted with REF were identified by yeast two-hybrid screening method, including two of REF family proteins, and small rubber particle protein (SRPP), translation control of tumor protein (TCTP), elicitor-responsive protein and ubiquitin-conjugating enzyme E2. The data suggested that REF could not only interact with high homologous proteins, but also with other proteins, such as TCTP, and E2, etc. These protein-protein interactions might help to revealing the biological function of H. brasiliensis rubber particles.

    Reference
    [1] Southorn W A. Complex particles in Hevea latex [J]. Nature, 1960, 188(4745): 165-166.
    [2] Dickenson P B. Electron microscopic studies of latex vessel system of Hevea brasiliensis [J]. J Rub Res Inst Malaya, 1969, 21(4): 543-559.
    [3] Kush A. Isoprenoid biosythesis: The Hevea factory [J]. Plant Physiol Biochem, 1994, 32(6): 761-767.
    [4] Ohya N, Koyama T. Biosynthesis of natural rubber and other natural polyisoprenoids [M]// Koyama T, Steinbuchel A. Biopolymers Volume 2: Polyisoprenoids. Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA, 2001: 73-81.
    [5] Dennis M S, Light D R. Rubber elongation factor from Hevea brasiliensis: Identification, characterization and role in rubber biosynthesis [J]. J Biol Chem, 1989, 264(31): 18608-18617.
    [6] Comish K. The separate roles of plant cis- and trans-prenyl transferases in cis-1,4-polyisoprene biosynthesis [J]. Eur J Biochem, 1993, 218(1): 267-271.
    [7] Oh S K, Kang H, Shin D H, et al. Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis [J]. J Biol Chem, 1999, 274(24): 17132-17138.
    [8] Duan C F(段翠芳), Nie Z Y(聂智毅), Zeng R Z(曾日中). Establishment of 2-DE system and primary analysis on the membrane proteins of rubber particles in Hevea brasiliensis by MALDI-TOF [J]. Chin J Trop Crops(热带作物学报), 2006, 27(3): 22-29.(in Chinese)
    [9] Chen S C(陈守才), Shao H S(邵寒霜), Hu D Q(胡东琼), et al. The relationship between the rubber transferase activity and the rubber-producing ability of rubber tree [J]. Chin J Trop Crops(热带作物学报), 1994, 15(3): 1-6.(in Chinese)
    [10] Priya P, Venkatachalam P, Thulaseedharan A. Differential expression pattern of rubber elongation factor (REF) mRNA transcripts from high and low yielding clones of rubber tree (Hevea brasiliensis Muell.-Arg.) [J]. Plant Cell Rep, 2007, 26(10): 1833-1838.
    [11] Zeng R Z, Duan C F, Li X Y, et al. Vacuolar-type inorganic pyrophosphatase located on the rubber particle in the latex is an essential enzyme in regulation of the rubber biosynthesis in Hevea brasiliensis [J]. Plant Sci, 2009, 176(5): 602-607.
    [12] Kim I J, Ryu S B, Kwak Y S, et al. A novel cDNA from Parthenium argentatum Gray enhances the rubber biosynthetic in vitro [J]. J Exp Bot, 2004, 55(396): 377-385.
    [13] Fields S, Song O. A novel genetic system to detect protein-protein interaction [J]. Nature, 1989, 340(20): 245-246.
    [14] Chien C T, Bartel P L, Sternglanz R, et al. The two-hybrid system: A method to identity and clone genes for proteins that interact with a protein of interest [J]. Proc Natl Acad Sci USA, 1991, 88(21): 9578-9582.
    [15] Miller J, Stagljar I. Using the yeast two-hybrid system to identify interacting proteins [J]. Methods Mol Biol, 2004, 261: 247-262.
    [16] Deng Z(邓治), Zhang Y X(张云霞), Chen C L(陈春柳), et al. Screening of the protein interaction with HbMyb1 using yeast two-hybrid system [J]. Plant Physiol Commun(植物生理学通讯), 2007, 43(6): 1031-1034.(in Chinese)
    [17] Zeng R Z(曾日中), Duan C F(段翠芳), Li Y(黎瑜), et al. Construction of the SSH library of latex cDNA and sequence analysis of JA-stimulated rubber tree [J]. Chin J Trop Crop(热带作物学报), 2003, 24(3): 1-6.(in Chinese)
    [18] Dennis M S, Henzel W J, Bell J, et al. Amino acid sequence of rubber elongation factor protein associated with rubber particles in Hevea latex [J]. J Biol Chem, 1989, 264(31): 18618-18628.
    [19] Wu K X(吴坤鑫), Wang Z(王震), Yao M P(姚茂平), et al. Comparative study of electrophoretic patterns of rubber particles from different clones of Hevea brasiliensis [J]. Anhui Agri Sci(安徽农业科学), 2008, 36(36): 15785-15787, 15803. (in Chinese)
    [20] Ohya N, Tanaka Y, Wititsuwannakul R, et al. Activity of rubber transferase and rubber particle size in Hevea latex [J]. J Rubber Res, 2000, 3(4): 214-221.
    [21] Sturzenbaum S R, Kille P, Morgan A J. Identification of heavy metal induced changes in the expression patterns of the translationally controlled tumor protein (TCTP) in the earthworm Lumbricus rubellusl[J]. Biochim Biophys Acta, 1998, 1398(3): 294-304.
    [22] Yarm F R. Plk phosphorylation regulates the microtubule-stabilizing protein TCTP [J]. Mol Cell Biol, 2002, 22(17): 6209-6221.
    [23] Mak C H, Poon M W, Lun H M, et al. Heat-inducible translationally controlled tumor protein of Trichinella pseudospiralis: Cloning and regulation of gene expression [J]. Parasitol Res, 2007, 100(5): 1105-1111.
    [24] Berkowitz O, Jost R, Pollmann S, et al. Characterization of TCTP, the translationally controlled tumor protein, from Arabidopsis thaliana [J]. Plant Cell, 2008, 20(12): 3430-3447.
    [25] Liang X L(梁小莲), Li H L(李辉亮), Peng S Q(彭世清). Cloning and expression of HbTCTP from Hevea brasiliensis [J]. Mol Plant Breed(分子植物育种), 2009, 7(1): 188-193.(in Chinese)
    [26] Chen S, Peng S, Huang G, et al. Association of decreased expression of a Myb transcription factor with the TPD (tapping panel dryness) syndrome in Hevea brasiliensis [J]. Plant Mol Biol, 2002, 51(1): 51-58.
    [27] Benschop J J, Mohamme S, O’Flaherty M, et al. Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis[J]. Mol Cell Proteome, 2007, 6(7): 1198-1214.
    [28] Xu M J(徐茂军), Dong J F(董菊芳), Zhu M Y(朱睦元). Stimulatory effects of NO-mediated fungal elicitors through SA- or JA-signaling on the puerarin biosynthesis in the suspension cells of root of kudzuvine [J]. Sci China Ser C: Life Sci (中国科学C辑: 生命科学), 2006, 36(1): 66-75.(in Chinese)
    [29] Hao B Z, Wu J L. Laticifer differentiation in Hevea brasiliensis: Induction by exogenous jasmonic acid and linolenic acid [J]. Ann Bot, 2000, 85(1): 37-43.
    [30] Bader M, Steller H. Regulation of cell death by the ubiquitin- proteasome system [J]. Curr Opin Cell Biol, 2009, 21(6): 878-884.
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曾日中,李先昆,聂智毅,代龙军,黎瑜,段翠芳.应用酵母双杂交系统筛选橡胶延长因子REF的互作蛋白[J].热带亚热带植物学报,2011,19(5):400~406

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History
  • Received:January 19,2011
  • Revised:April 06,2011
  • Adopted:June 14,2011
  • Online: September 27,2011
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