Abstract:The isolation and screening pacilitaxel-producing endofungus from Taxus chinensis var. mairei were studied, which derived from Ruyuan Xian, Guangdong Province. The 145 endophytic fungi were obtained from the bark, stem, leaf and leaf grinding juice of T. chinensis var. mairei, respectively, in which 53 strains were fermented by shake flask for screening paclitaxel-producing endophytic fungi. The fermented solutions and thalli were grinded, centrifugated, extracted by ethyl acetate and concentrated, and one strain of endophytic fungal from the shem of T. chinensis var. mairei was identified by using thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and HPLC-mass spectrometry (MS). The strain of endofungus could produce paclitaxel or its isimer with yield of 180 μg L-1. Thus, It should be possible for increasing the yield of paclitaxel on a large scale by mutagenesis, screening and optimizing the culture condition.