Studies on cry1Ac Gene Transgenic Plants of Saccharum Complex by Particle Bombardment

Abstract:

Plant expression vectors pUBCG0229-cry1Ac with size of 8602 bp, which had anti-insect gene and the bar gene as screening marker gene, was constructed. The result of restriction digestion showed that the construction of the vectors pUBCG0229-cry1Ac was completely correct. The embryonic callus of Saccharum Complex ‘FN95-1702’ and ‘GT94-119’ were transformed with the plasmid of pUBCG0229-cry1Ac by bombardment. After selection with PPT (Phosphinothricin) or Basta, 86 regenerated plants were obtained. After detected by PCR, Dot-Southern blotting and sequencing of PCR products from 22 transformed plants, it proved that cry1Ac gene had been integrated into sugarcane genome.

Key words:Sugarcane;cry1Ac) gene;bar gene;Genetic transformation;Gene gun;Resistance to Chi1o spp.