Abstract:Four steroids were isolated from the fermented mycelia of F. rabenhorstii A20, an endophytic fungus from Aquilaria sinensis (Lour.) Gilg. On the basis of spectral data, they were identified as 5α,8α-epidioxy-(22E,24R)-ergosta-6,22-dien-3β-ol (1), 3β,6β,7α-trihydroxy- (24R)-ergosta-8(14),22-diene (2), 3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (3), and 3β,5α,6β-trihydroxy-(22E,24R)-ergosta-7,22-diene (4). All compounds were isolated from the genus Fimetariella for the first time.