Abstract:Eight compounds were isolated from the ethanol extract of Lycopodium japonicum. On the basis of spectral data, they were identified as 3β,21β,24-trihydroxyserrat-14-en-16-one (1), 3α,21β,24-trihydroxyserrat-14-en-16-one (2), 3β,21β,24-trihydroxyserrat-14-en (3), 3α,21β,24-trihydroxyserrat-14-en (4), onocerin (5), stigmasta-5-en-3β,7α-diol (6), stigmasta-5-en-3β,7β-diol (7), β-sitosterol (8). Compound 1 was a new compound, and compounds 3, 4, 6, 7 were obtained from this species for the first time, and the spectral data of compound 2 was reported for the first time.