Extraction of Total Protein from Litchi Pericarps and Establishment of Two-dimensional Electrophoresis
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    Abstract:

    The total protein was extracted from Lichi chinensis pericarp by using three methods, such as trichloroacetic acid (TCA)-acetone, acetone and phenol. The results showed that the total protein yield was the highest by phenol extraction method, and protein bands were the most and clear in one dimentional SDS-PAGE. About 870 clear protein spots were detected by two-dimensional electrophoresis (2-DE) and silver staining with isoelectric points at pH 4~8, and relative molecular weight of protein ranged from 15.0 to 85.0 kD.

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李开拓,郭志雄,潘东明,钟凤林,潘腾飞.荔枝果皮总蛋白质提取及双向电泳体系的建立[J].热带亚热带植物学报,2011,19(1):69~74

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History
  • Received:March 30,2010
  • Revised:August 22,2010
  • Adopted:September 06,2010
  • Online: January 25,2011
  • Published: