Abstract:The differential proteins of floral reversion buds at different stages in longan (Dimocarpus longan Lour.) were compared using proteomics method. The results showed that α-tubulin could up-regulate in floral reversion buds of longan, and its gene α-tubulin was cloned using RACE method. A full length of 1641 bp cDNA, with a 1350 bp open reading frame, was obtained (GenBank accession number: FJ479617). When α-tubulin gene was transformed into E. coli and expressed, a 49.6 kD heterologous protein verified as α-tubulin by Western blotting was obtained. Different expression of α-tubulin at transcription and translation levels using RT-PCR and Western blotting, respectively, showed that α-tubulin up-regulated in longan floral reversion buds. It was one of reasons that floral reversion buds were different from normal flowering buds.
