Abstract:The aqueous extract from the whole plant of Isodon lophanthoides var. graciliflora was separated and purified by means of chromatographic technology and eleven compounds were isolated. On the basis of spectral data, they were identified as vitexin (1), pachypodol (2), chrysoplenetin (3), 9-hydroxy-5,7-megastigmadien-4-one (4), 3,9-dihydroxy-5,7-megastigmadien-4-one (5), loliolide (6), 4-acetyl-5-methyldihydrofuran-2-one (7), 5-hydroxymethylfuraldehyde (8), 5-methoxymethyl-1H-pyrrole-2-carbaldehyde (9), indole-3-aldehyde (10), and succinic acid monobutyl ester (11). All the compounds were obtained from Isodon lophanthoides for the first time.