EExpression Analysis of OsMATE in Rice under Abiotic Stresses

Abstract:

OsMATE, the closest homology of sorghum (Sorghum bicolor) SbMATE, was cloned by PCR technique from rice (Oryza sativa) leaves, and then its expression pattern was analysed in different tissues and under abiotic stresses by semi-quantitative RT-PCR. The result showed that OsMATE expressed lowly in rice roots and leaves, and almost none in leaf sheaths. OsMATE expressed strongly in rice roots induced by abiotic stresses, such as aluminum (Al), cadmium, arsenic, salt, iron, PEG6000, paraquat and ABA, while little expressed in leaves by arsenic, salt and PEG6000 stress. The expression of OsMATE in roots of the Al-resistant ‘XN1’ was more than that in the Al-susceptible ‘XX2’ under Al stress. It suggested that OsMATE could play an important role in stress resistance of rice under abiotic stresses.

Key words:Rice; OsMATE; Semi-quantitive RT-PCR; Abiotic stresses