The effects of storage conditions on pollen germination of Casuarina cunninghamiana Miq. in vitro were studied. The results showed that the optimum concentrations of sucrose and boric acid on pollen germination of C. cunninghamiana Miq. were 15% and 250 mg kg-1, respectively. The solid medium supplemented with agar was beneficial to pollen germination. Pollen at low temperature could maintain germination ability for long time.