Abstract:Chirita luzhaiensis Yan Liu Y. S. Huang & W. B. Xu, a new species of Gesneriaceae from limestone areas in Guangxi, China, is described and illustrated. The new species is similar to Chirita gueilinensis W. T. Wang, but it differs in its leaf-surface villous and pubescent adaxilly, anthers glabrous, filaments geniculate near base, staminodes 3, glabrous, and flowering period from December to January of the following year. It was only found in two karst caves from Zhongdu Town of Luzhai County, Guangxi, China.