Abstract:The phytoactivity of toal alkaloids from stem of Cyperus iria to Oryza sativa, Nicotiana tabacum, Plantago asiatica, Eclipta prostrate, Leptochloa chinensis and antifungal activity against Magnaporthe grisea, Sclerotinia sclerotiovum, Alternaria solani, Fusarium graminearum, Dothiorella gregaria and Physalospora piricola were measured. The results showed that 250~4 000 μg mL-1 total alkaloids had not significant influence in seed germination of O. sativa, but significantly inhibited in seedling height of L. chinensis. Seed germination of N. tabacum, E. prostrate and P. asiatica, seedling height of N. tabacum, seedling fresh weight of O. sativa, N. tabacum and E. prostrate were significantly inhibited treated with 4 000 μg mL-1 total alkaloids. The total alkaloids of low concentrations significantly inhibited in seed germination of L. chinensis, seedling fresh weight of L. chinensis and P. asiatica, seedling root length and height of five species except of seedling height of N. tabacum. However, 25~400 μg mL-1 total alkaloids had no significant inhibition to the growth of F. graminearum, but had to the growth of P. piricola, and 100~400 μg mL-1 total alkaloids had significant inhibition to M. grisea, S. sclerotiovum and A. solani, while did 200~400 μg mL-1 total alkaloids to D. gregariaat, the growth of six fungi were significantly inhibited treated with 400 μg mL-1 total alkaloids, the inhibitory rates were 8.20%, 74.87%, 40.93%, 60.91%, 52.70% and 16.28%, respectively.