YOU Xiang-rong
Key Laboratory of Crop Genetics and Multipurpose Utilization, Ministry of Education, Fujian Agriculture and Forestry University; College of Life Science, Fujian Agriculture and Forestry UniversityXU Hong-chuan
College of Life Science, Fujian Agriculture and Forestry UniversityLIANG Wen-yu
College of Life Science, Fujian Agriculture and Forestry UniversityCHEN Qing-xi
College of Horticulture, Fujian Agriculture and Forestry UniversityZHENG Shao-quan
Fruit Research Institute, Fujian Academy of Agriculture ScienceCHEN Wei
Key Laboratory of Crop Genetics and Multipurpose Utilization, Ministry of Education, Fujian Agriculture and Forestry University; College of Life Science, Fujian Agriculture and Forestry UniversityThe changes in proteome of normal flower and floral reversion buds in longan (Dimocarpus longan Lour.) were studied by using Proteomics method. The results showed that ANS (anthocyanidin synthase) protein was found down-regulated expression in floral reversion buds. ANS cDNA was obtained (GenBank access number: FJ479616) by using RACE method, its full length was 1477 bp, with a 1071 bp open read frame. ANS gene was expressed in E. coli, and a 46 kD heterologous protein was obtained. It suggested that ANS protein play a role in floral reversion in longan.
游向荣,许鸿川,梁文裕,陈清西,郑少泉,陈伟.龙眼花芽ANS基因的克隆与原核表达[J].热带亚热带植物学报,2010,18(3):288~292
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