Cloning and Sequence Analysis of Expansin gene from Cambium of Anthocephalus chinensis

Abstract:

Using the cDNA from the branch cambium of Anthocephalus chinensis as the template, the degenerate primers by reference to the α-Expansin gene (EXPA) sequences in other plants were constructed for RT-PCR. Taking the production of the amplification as the starting information, The full-length cDNA of expansin gene was obtained by RACE, named as AcEXP1. It was 979 bp in length (GenBank accession No. FJ417847) with 777 bp ORF, encoding 258 amino acid, which had the typical conserved sequence and domain of Expansin. There were 85%, 85%, 84%, 83% homology with Populua tomentosa, Populus tremula×P. tremuloides, Prunus cerasus, Petunia×hybrida, respectively. It provided the foundation for studying the relationships between EXP gene and growth rate, wood quality of the xylem of Anthocephalus chinensis.

Key words:Anthocephalus chinensis;Expansin;RACE;AcEXP1