Obtaining the GAI Transgenic Plants of Hevea brasiliensis by Particle Bombardment
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    Abstract:

    The vector pBI121 particle, which contains Camv35S promoter, kanamycin resistance gene, GUS reporter gene and GAI gene, was transfered into the Hevea brasiliensis anther callus by particle bombardment. The regenerated plantlets were obtained in the subculture media supplemented with kanamycin of 50 mg L-1. PCR and Southern Blotting demonstrated that the GAI gene was transferred into the H. brasiliensis genome successfully.

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洪磊,王颖,陈雄庭,张秀娟.基因枪法获得GAI转基因橡胶植株的研究[J].热带亚热带植物学报,2010,18(2):165~169

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History
  • Received:March 25,2009
  • Revised:November 30,2009
  • Adopted:December 03,2009
  • Online: March 22,2010
  • Published:
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