Obtaining the GAI Transgenic Plants of Hevea brasiliensis by Particle Bombardment

Abstract:

The vector pBI121 particle, which contains Camv35S promoter, kanamycin resistance gene, GUS reporter gene and GAI gene, was transfered into the Hevea brasiliensis anther callus by particle bombardment. The regenerated plantlets were obtained in the subculture media supplemented with kanamycin of 50 mg L-1. PCR and Southern Blotting demonstrated that the GAI gene was transferred into the H. brasiliensis genome successfully.

Key words:Hevea brasiliensis; Particle Bombardment;GAI gene