Differential Expression Analysis of Longan Cotyledon Embryo by cDNA-AFLP
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    Abstract:

    The finger map of longan (Dimdcarpus longan Lour. ‘Honghezi’) cotyledon embryo RNA was established by cDNA-AFLP, which extracted from thirty-five-day (early) and fifty-day (late) cotyledon embryos after anthesis, respectively. There were 41 differential fragments cloned and sequence analysed, in which 21 differential expression fragments had high homology with some known genes, the function mainly involved in the abaxial polarity of lateral organ, glycolysis, energy metabolism, ion transport, cell wall elongation, cell cycle regulation, RNA transcription, RNA translation and regulation, protein phosphorylation regulation and protein degradation, signal transduction and so on. While the others had low or no homology with known genes.

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卢秉国,申艳红,蒋际谋,陈晓静,陈伟,吕柳新.利用cDNA-AFLP技术分析龙眼子叶胚基因表达差异[J].热带亚热带植物学报,2009,17(6):562~566

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History
  • Received:January 15,2009
  • Revised:May 06,2009
  • Adopted:June 08,2009
  • Online: November 25,2009
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