Abstract:Plant regeneration through callus induction were established by using young sheath of fruit sugarcane (Saccharum officenarum ‘Badila’), and the transformation of calli mediated by Agrobacterium tumefaciens was studied. The optimum media were MS + 2.0 mg L-1 2,4-D for calli induction, MS + 2,4-D 2.0 mg L-1 + 6-BA 1 mg L-1 for differentiation; and MS + IAA 2.0 mg L-1 for root induction, respectively. Plant expression vector pMG225, including cryIA gene and CPTI gene, was transformed into calli of fruit sugarcane by Agrobacterium tumefaciens. It confirmed that cryIAc gene had been integrated into fruit sugarcane genome by PCR and Southern blot analysis.
