Abstract:The silent information regulator protein (Sir2) and its homologues collectively known as Sirtuins, NAD+-dependent deacetylase enzymes, play an important role in physiological regulation, such as the survival, aging and apoptosis of cell. The biological function of SIR2 HDACs family from Magnaporthe grisea was investigated by using bioinformatics. The results showed that the members of SIR2 family were localized in the different chromosomes and different parts of Magnaporthe grisea cell, and their encoding proteins all have four sites, such as the protein kinase C phosphorylation site, the Casein kinase II phosphorylation site, the N-glycosylation site, and the N-myristoylation site. The SIR2 HDACs members all haven’t transmembrane regions and they are hydrophilic proteins without signal peptide. The SIR2 HDACs members from M. grisea were aligned by ClustalW2 and their SIR2 domains are highly conserved. These SIR2 HDACs seemed to be involved in processes, such as transcription regulation, energy metabolism and chromatin silencing in M. grisea.