Abstract:Two hundred and fifty-eight bryophyte samples were collected from Guizhou Lannigou Gold Deposit which is the biggest carlin gold deposit in Asia. The bryoflora of this site included 15 families, 37 genera and 81 species. Pottiaceae, Bryaceae, Ditrichaceae and Bartramiaceae are four dominant families, which were adapted to their environment. Contents of Au, Cu, Zn, Pb, Cd, Cr, Co, As and Hg in 6 bryophytes [Pylaisiella polyantha, Bryum pallescens var. pallescens, Ditrichum heteromallum, Weisia planifolia, Weisia semipallida, and Cyrto-hypnum versicolor] and corresponding substrates were determined by using Atomic Absorption Spectrometer and Atomic Fluorescence Spectrometer. The results showed that the average concents of As and Hg in soil are 222.378 mg kg-1 and 23.516 mg kg-1, respectively, which are 5.56 times and 15.68 times of the third class standard (40 mg kg-1 for As, 1.5 mg kg-1 for Hg) of National Environmental Quality Standard for Soil. The pollution situation is serious. In addition, Bioconcentration factor show that each species of bryophyte accumulate 3 kind of heavy metals at least. Therefore, all of 6 species in this study can be used in mines polluted by heavy metals as pioneer plants for ecological restoration in further research.