Sterilization of the Explant and Callus Induction of Feijoa sallowiana Berg. in vitro Culture

Abstract:

The optimum conditions for the tissue culture of Feijoa (Feijoa sallowiana Berg.) were investigated. The leaves and shoot with bud were used as explants. The results showed that the optimal basal medium was MS medium. Two-step sterilization was better than one-step sterilization. The optimal concentration of active carbon for callus induction from leaves and shoot with bud were 2.0 g L-1 and 3.0 g L-1, respectively, and penicillin were 200 mg L-1 and 50 mg L-1, respectively. The culture under light was better than under dark. The optimal medium for callus induction from tender leaves was MS+2.0 mg L-1 2,4-D+1.0 mg L-1 KT (or 0.1 mg L-1 NAA), and from shoot with bud was MS+1.0 mg L-1 6-BA+0.5mg L-1 NAA+1.0 mg L-1 GA, or MS+2.0 mg L-1 6-BA+0.1 mg L-1 NAA+1.0 mg L-1 GA.

Key words:Feijoa sallowiana Berg.; Tissue culture; Leaf; Shoot with bud