Abstract:The effects of sucrose, boric acid, calcium, pH, and illumination on the fresh pollen germination of Lonicera maackii (Rupr.) Maxim. were studied. The results shown that the pollen germination rate were 85.63%, 59.23%, and 78.12%, when medium supplied with 25% sucrose, 0.01% boric acid, 0.02%~0.05% Ca(NO3)2, respectively, and the germination rate got 75.27％ cultured under pH 6.7. The effects of carbohydrates on pollen germination rate in order were sucrose＞glucose＞lactose ＞fructose. There was not significant difference of pollen germination rate under illumination (0.5625 μmol m-2s-1) and dark, but the length of pollen tube was longer under the dark than under the illumination. The optimal medium for pollen germination consist of 25% sucrose, 0.01% boric acid, 0.02% Ca(NO3)2 at pH 6.7 and under illumination, in which the pollen germination rate reached 88.1％.
