Abstract:The genomic DNA was extracted from Castanopsis hystrix A. DC. using the modified method of CTAB, and the optimization of its RAPD reaction system was also studied. Results showed that the high-quality genomic DNA can be obtained with the modified method of CTAB, and it could be directly used for RAPD analyses. The optimal PCR system for RAPD analysis was as follows: 0.8 ng μl-1 DNA template, 2.0 mmol/L Mg2+, 0.8 U Taq polymerase, 0.35 mmol/L dNTPs, 0.28 μmol/L random primer, in 25 μl reaction system. RAPD program is 3 minutes at 94℃ for predenaturation, then followed by 35 cycles, each with 30 seconds at 94℃ for denaturation, 1minute at 39℃ for annealing , 2 minutes at 72℃ for extension, finally extension at 72℃ for 10 minutes.
