Abstract:he oils extracted from Jatropha curcas seeds at different ripening and storage stages were analyzed by GC-MS. The physicochemical characteristics of the seed oils were also determined. The chemical composition of the seed oils, iodine value, and saponification value were similar, whereas the yields, acid values and water content of the seed oils showed obvious differences. The yield, water content and acid value of the seed oil from the green fruit were 13.31%, 0.66% and 69.21, respectively, while those from the fresh ripe fruits were 54.64%, 0.36%, and 1.51, respectively. The content of unsaturated fatty acids in the seed oils of green fruits was lowest. Therefore, the fresh ripe fruits are more preferable for raw material of Bio-diesel oil.