Abstract:Four antifungal protein genes (RAC22、RCH10、β-1,3-Glu and B-Rip) were introduced into Indica hybrid rice (Oryza sativa L.) restorer line E32 by particle bombardment. Ten transgenic progenies of T4 generation exhibited resistance to sheath blight after inoculated at the active tillering stage. In addition, the transgenic progenies had obvious variations in some non-target agronomic traits, such as growth duration, morphology, grain appearance and grain yield. The genetic gains were different among transgenic lines and the traits. Panicle, grain density and grain yield per plant had the highest genetic gains. The variation coefficients of plant height, panicle length and grain appearance were low, while those of panicle numbers per plant and 1000-grain weight reached high levels. The transgenetic rice cultivars with high yield and good quality could be developed.