Abstract:We investigated influences of white light, UV-B, red light and blue light on phenylalanine ammonia-lyase (PAL) activity, baicalin content and cell growth in suspension culture of Scutellaria baicalensis Georgi. PAL activity and baicalin accumulation were promoted significantly by UV-B, moderately by white and blue lights. Red light had negative effects on PAL activity and baicalin accumulation. Baicalin content and PAL activity remarkably increased with UV-B intensity rising from 0 to 60 μmol m-2 s-1. The cell growth was promoted by red light, inhibited by UV-B, and unaffected by blue and white lights. Following treated with the alternation of 8 h UV-B of 60 μmol m-2 s-1 and 16 h red light of 50μmol m-2 s-1, fresh cell weight and baicalin content were 1.16- and 3.2-fold, respectively, higher than the controls. The yield of the total baicalin was 439 mg L-1 which was 3.8-fold higher than controls.
