Abstract:Viability of pollen of Magnolia soulangeana was tested at different storage temperatures and methods. Culture solution of 5% sucrose supplemented with 0.01% or 0.1% boric acid gave good results for pollen germination, the germination rate reaching to above 70%. Pollen viability declined with the increased preservation time. The decrease of pollen viability was slowered in the order of room temperature, 5℃, and -20℃. Cryogenic storage at -196℃ had no significant changes, even though the pollen stored for 2 years, the germination rate being 79.3%. Pollen samples exhibited high tolerance to re-freezing and thawing.
