Abstract:GAI gene was transferred to calli from anther culture ofHevea brasiliensis by particle bombardment to develop dwarf rubber trees. Plant material was bombarded with plasmid PBI121 containing kanamycin resistant, GUS and GAI genes. Kanamycm concentration at 50 mg L-1 was used as selection pressure alter concentration gradient experiments. The experiments showed that each plate of Hevea calli, which was bombarded once by gold particles coated with the plasmid and the distance between particles and target cells at 9 cm, had good results, the embryoid induction rate reaching to 1.87%. It was confirmed that GAI gene had been inserted into Hevea genome as detected by histochemical staining using X-gluc and PCR analysis.
