Rapid Propagation of Cymbidium kanran Makino

Abstract:

Propagation of Cymbidium kanran Makino by using rhizomes as explants to study the effects of growth regulators on the induction, multiplication and differentiation of protocorm-like bodies （PLBs）, and the rooting. The results showed that the optimal medium for induction of PLBs was B₅ containing 0.50 mg L^-1 TDZ and 0.25 mg L^-1 NAA, in which induction rate of PLBs was 98.3%, for multiplication of PLBs it was B₅ containing 1.0 mg L^-1 uniconazole （S-3307） and 0.2 mg L^-1 NAA and 3.5% sucrose, the multiplication coefficient being 9.4. For differentiation it was best on B₅ medium containing 0.75 mg L^-1 S-3307, 1.0 mg L^-1 6-BA and 0.4 mg L^-1 NAA, in which the differentiation rate was 87.8%. As for rooting, 1/2B₅ supplemented with 0.2 mg L^-1 NAA and 0.05% active carbon, in which rooting rate reached 100%.