Crude proteins from green leaves ofBrassica campestris L. ssp. chinensis （L.） Makino var. utillis Tsen et Lee and Lactuca sativa L. were subjected to acetate cellulose membrane electrophoresis under SDS-denatured conditions. Two groups of protein were observed, one migrated towards the anode, and the other, the cathode. The cathode protein from Brassica campestris ssp. chinensis vat. utillis was collected from cathode reservoir after SDS-PAGE, and its amino acid components were determined to have 57.0% phenylalanine, the ratio of basic/ acidic amino acid residues was 3.04, indicating the protein towards cathode was a phenylalanine rich basic protein. It must be emphasized that the protein migrated towards the cathode had never been reported previously.
